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KMID : 1024520070160121337
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Journal of the Environmental Sciences
2007 Volume.16 No. 12 p.1337 ~ p.1343
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Isolation and Characterization of a Feather-Degrading Bacterium for Recycling of Keratinous Protein Waste
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Kim Jung-Chul
Kim Min-Ju
Son Hyeng-Sik
Ryu Eun-Youn
Jeong Seong-Yun
Kim Mi-A
Park Geun-Tae
Son Hong-Joo
Lee Sang-Joon
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Abstract
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The aim of this study was to isolate chicken feather-degrading bacteria with high keratinolytic activity and to investigate cultural conditions affecting keratinolytic enzyme production by a selected isolate. A chicken feather-degrading bacterial strain CH3 was isolated from poultry wastes. Isolate CH3 degraded whole chicken feather completely within 3 days. On the basis of phenotypical and 16S rDNA studies, isolate CH3 was identified as Bacillus thuringiensis CH3. This strain is the first B. thuringiensis described as a feather degrader. The bacterium grew with an optimum at pH 8.0 and , where maximum keratinolytic activity was also observed. The composition of optimal medium for keratinolytic enzyme production was feather 0.1%, sucrose 0.7%, casein 0.3%, 0.03%, 0.04%, 0.01% and NaCl 0.05%, respectively. The keratinolytic enzyme had a pH and temperature optima 9.0 and , respectively. The keratinolytic activity was inhibited ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, and metal ions like . The enzyme activated by , dithiothreitol and 2-mercaptoethanol.
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KEYWORD
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Feather, Keratin, Keratinolytic enzyme, Waste
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